Sliding-end-labelling
نویسندگان
چکیده
منابع مشابه
DNA orientation using specific avidin-ferritin biotin end labelling.
The orientation of DNA molecules has been determined by labelling one of the molecule end with a Biotin-labelled analog of dTTP (Bio-dUTP) and then by complexing the Bio-dUTP with Avidin-Ferritin. DNAs of phi X174, pBR322 and SV40 were end labelled with Bio-dUTP and imaged by Electron Microscopy (EM). This is a rapid, general method to unambiguously determine the orientation of DNA molecules fo...
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Background Chlamydia trachomatis is considered as an important cause of preventable sexually transmitted diseases worldwide. It is known to be of an obligate intracellular nature and enters its target cells via an endocytic process. As major outer membrane protein (MOMP) is one of the main candidates for the attachment and entry of chlamydia to the host cells we have tried to label the epitopes...
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In computational models of argumentation, argument justification has attracted more attention than statement justification, and significant sensitivity losses are identifiable when dealing with the justification of statements by otherwise appealing formalisms. This paper reappraises statement justification as a formalism-independent component in argument-based reasoning. We introduce a novel ge...
متن کاملIn situ end labelling: effect of proteolytic enzyme pretreatment and hydrochloric acid.
AIM To determine the effect of dilute hydrochloric acid on the in situ end labelling (ISEL) reaction, with and without a variety of different proteolytic enzymes. METHODS Sections of tissue fixed in buffered formalin were pretreated with trypsin, protease XIV (at two different concentrations), or protease XXIV (for two different incubation times), with and without subsequent 1 M hydrochloric ...
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One drawback when using non-radioactive labelling has been that there is no easy test for the degree of incorporation of nucleotide analogues. Here we show that biotinylated DNA-fragments have a lower mobility than non-biotinylated fragments during agarose gel electrophoresis, and that this gives a good indication of the degree of end-labelling of DNA-fragments. This feature can be exemplified ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1986
ISSN: 0014-5793
DOI: 10.1016/0014-5793(86)81525-3